Zonal rotor study of the subcellular distribution of acyl CoA synthetases, carnitine acyl transferases and phosphatidate phosphatase in the guinea pig small intestine

P. A. Martin, N. J. Temple, M. J. Connock

Research output: Contribution to journalJournal Articlepeer-review

8 Citations (Scopus)

Abstract

Homogenates made from the mucosa of the guinea pig small intestine were fractionated in a zonal rotor by rate and isopyenic centrifugation in sucrose gradients. Density perturbation of endoplasmic reticulum vesicles was done by treating homogenate with pyrophosphate and was analysed by isopyenic centrifugation. Subcellular fractions were analysed for the distribution of markers for brush borders, basolateral plasma membrane, lysosomes, peroxisomes, mitochondria, nuclei and endoplasmic reticulum. Fractions were also analysed for the distribution of propionyl-, butyryl-, and palmityl-CoA synthetases. For carnitine acetyl and palmityl transferases, and for phosphatidate phosphatase. Comparison of marker and unknown distributions shows that palmityl-CoA synthetase is located on the endoplasmic reticulum, while propionyl- and butyryl-CoA synthetases and carnitine acetyl and palmityl transferase are exclusively mitochondrial. Phosphatidate phosphatase has complex subcellular localisation with activity in brush borders, microsomes (possibly not the endoplasmic reticulum component) and possibly lysosomes.

Original languageEnglish
Pages (from-to)3-10
Number of pages8
JournalEuropean Journal of Cell Biology
Volume19
Issue number1
Publication statusPublished - 1979

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