Abstract
Whele homogenate of guinea-pig small intestine mucosa was analysed by centrifugation in a zonal rotor. The results indicate that FAD-linked α-glycerophosphate dehydrogenase is localized in the mitochondria and that NAD-linked α-glycerophosphate dehydrogenase is a soluble phase enzyme. An enzyme hydrolysing α-naphthyl palmitate at an acid pH was localized in the lysosomes and was activated by 0.1% Triton X-roo and by freezing and thawing. An alkaline hydrolase acting on β-naphthyl laurate was localized in the 'microsomes'. The possibility of this enzyme being different from α-naphthyl acetate hydrolase is discussed.
Original language | English |
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Pages (from-to) | 159-175 |
Number of pages | 17 |
Journal | Histochemical Journal |
Volume | 8 |
Issue number | 2 |
DOIs | |
Publication status | Published - Mar. 1976 |