Zonal rotor analysis of the subcellular localization of α-glycerophosphate dehydrogenase, α-naphthyl palmitate and β-naphthyl laurate hydrolases in the mucosa of the guinea-pig small intestine

Norman J. Temple, Patricia A. Martin, Martin J. Connock

Research output: Contribution to journalJournal Articlepeer-review

4 Citations (Scopus)

Abstract

Whele homogenate of guinea-pig small intestine mucosa was analysed by centrifugation in a zonal rotor. The results indicate that FAD-linked α-glycerophosphate dehydrogenase is localized in the mitochondria and that NAD-linked α-glycerophosphate dehydrogenase is a soluble phase enzyme. An enzyme hydrolysing α-naphthyl palmitate at an acid pH was localized in the lysosomes and was activated by 0.1% Triton X-roo and by freezing and thawing. An alkaline hydrolase acting on β-naphthyl laurate was localized in the 'microsomes'. The possibility of this enzyme being different from α-naphthyl acetate hydrolase is discussed.

Original languageEnglish
Pages (from-to)159-175
Number of pages17
JournalHistochemical Journal
Volume8
Issue number2
DOIs
Publication statusPublished - Mar. 1976

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