Subcellular localization of monoglyceride acyltransferase, xanthine oxidation, NADP: Isocitrate dehydrogenase and alkaline phosphatase in the mucosa of the guinea-pig small intestine

P. A. Martin; N. J. Temple; M. J. Connock

doi:10.1016/0305-0491(80)90152-2

Subcellular localization of monoglyceride acyltransferase, xanthine oxidation, NADP: Isocitrate dehydrogenase and alkaline phosphatase in the mucosa of the guinea-pig small intestine

P. A. Martin
, N. J. Temple
, M. J. Connock

Centre for Science

University of Wolverhampton

Research output: Contribution to journal › Journal Article › peer-review

3 Citations (Scopus)

Abstract

1. 1. Rate dependent and isopycnic banding in a zonal rotor were used to analyse the subcellular sites of enzymes in homogenates of guinea-pig small intestinal mucosa. 2. 2. The results demonstrate the following localizations: monoglyceride acyltransferase-microsomal; xanthine oxidase and dehydrogenase-soluble phase, and NADP: isocitrate dehydrogenase-soluble phase and mitochondrial. 3. 3. Alkaline phosphatase is confined to brush borders and is absent from the basolateral plasma membrane. A variable proportion of the activity, up to 40% is on brush borders which during homogenization break up into particles of reduced density and slow sedimentation rate.

Original language	English
Pages (from-to)	327-332
Number of pages	6
Journal	Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology
Volume	67
Issue number	2
DOIs	https://doi.org/10.1016/0305-0491(80)90152-2
Publication status	Published - 1980

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10.1016/0305-0491(80)90152-2

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Martin, P. A., Temple, N. J., & Connock, M. J. (1980). Subcellular localization of monoglyceride acyltransferase, xanthine oxidation, NADP: Isocitrate dehydrogenase and alkaline phosphatase in the mucosa of the guinea-pig small intestine. Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology, 67(2), 327-332. https://doi.org/10.1016/0305-0491(80)90152-2

@article{78ed73d3bfe748369a9a53ebcfd68f67,

title = "Subcellular localization of monoglyceride acyltransferase, xanthine oxidation, NADP: Isocitrate dehydrogenase and alkaline phosphatase in the mucosa of the guinea-pig small intestine",

abstract = "1. 1. Rate dependent and isopycnic banding in a zonal rotor were used to analyse the subcellular sites of enzymes in homogenates of guinea-pig small intestinal mucosa. 2. 2. The results demonstrate the following localizations: monoglyceride acyltransferase-microsomal; xanthine oxidase and dehydrogenase-soluble phase, and NADP: isocitrate dehydrogenase-soluble phase and mitochondrial. 3. 3. Alkaline phosphatase is confined to brush borders and is absent from the basolateral plasma membrane. A variable proportion of the activity, up to 40\% is on brush borders which during homogenization break up into particles of reduced density and slow sedimentation rate.",

author = "Martin, \{P. A.\} and Temple, \{N. J.\} and Connock, \{M. J.\}",

note = "Funding Information: Acknowledgement We gratefully acknowledge the financial support of the Wellcome Trust.",

year = "1980",

doi = "10.1016/0305-0491(80)90152-2",

language = "English",

volume = "67",

pages = "327--332",

number = "2",

}

Martin, PA, Temple, NJ & Connock, MJ 1980, 'Subcellular localization of monoglyceride acyltransferase, xanthine oxidation, NADP: Isocitrate dehydrogenase and alkaline phosphatase in the mucosa of the guinea-pig small intestine', Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology, vol. 67, no. 2, pp. 327-332. https://doi.org/10.1016/0305-0491(80)90152-2

Subcellular localization of monoglyceride acyltransferase, xanthine oxidation, NADP: Isocitrate dehydrogenase and alkaline phosphatase in the mucosa of the guinea-pig small intestine. / Martin, P. A.; Temple, N. J.; Connock, M. J.
In: Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology, Vol. 67, No. 2, 1980, p. 327-332.

Research output: Contribution to journal › Journal Article › peer-review

TY - JOUR

T1 - Subcellular localization of monoglyceride acyltransferase, xanthine oxidation, NADP

T2 - Isocitrate dehydrogenase and alkaline phosphatase in the mucosa of the guinea-pig small intestine

AU - Martin, P. A.

AU - Temple, N. J.

AU - Connock, M. J.

N1 - Funding Information: Acknowledgement We gratefully acknowledge the financial support of the Wellcome Trust.

PY - 1980

Y1 - 1980

N2 - 1. 1. Rate dependent and isopycnic banding in a zonal rotor were used to analyse the subcellular sites of enzymes in homogenates of guinea-pig small intestinal mucosa. 2. 2. The results demonstrate the following localizations: monoglyceride acyltransferase-microsomal; xanthine oxidase and dehydrogenase-soluble phase, and NADP: isocitrate dehydrogenase-soluble phase and mitochondrial. 3. 3. Alkaline phosphatase is confined to brush borders and is absent from the basolateral plasma membrane. A variable proportion of the activity, up to 40% is on brush borders which during homogenization break up into particles of reduced density and slow sedimentation rate.

AB - 1. 1. Rate dependent and isopycnic banding in a zonal rotor were used to analyse the subcellular sites of enzymes in homogenates of guinea-pig small intestinal mucosa. 2. 2. The results demonstrate the following localizations: monoglyceride acyltransferase-microsomal; xanthine oxidase and dehydrogenase-soluble phase, and NADP: isocitrate dehydrogenase-soluble phase and mitochondrial. 3. 3. Alkaline phosphatase is confined to brush borders and is absent from the basolateral plasma membrane. A variable proportion of the activity, up to 40% is on brush borders which during homogenization break up into particles of reduced density and slow sedimentation rate.

UR - https://www.scopus.com/pages/publications/33646282262

U2 - 10.1016/0305-0491(80)90152-2

DO - 10.1016/0305-0491(80)90152-2

M3 - Journal Article

AN - SCOPUS:33646282262

SN - 0305-0491

VL - 67

SP - 327

EP - 332

JO - Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology

JF - Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology

IS - 2

ER -

Subcellular localization of monoglyceride acyltransferase, xanthine oxidation, NADP: Isocitrate dehydrogenase and alkaline phosphatase in the mucosa of the guinea-pig small intestine

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