Subcellular localization of monoglyceride acyltransferase, xanthine oxidation, NADP: Isocitrate dehydrogenase and alkaline phosphatase in the mucosa of the guinea-pig small intestine

P. A. Martin, N. J. Temple, M. J. Connock

Research output: Contribution to journalJournal Articlepeer-review

3 Citations (Scopus)

Abstract

1. 1. Rate dependent and isopycnic banding in a zonal rotor were used to analyse the subcellular sites of enzymes in homogenates of guinea-pig small intestinal mucosa. 2. 2. The results demonstrate the following localizations: monoglyceride acyltransferase-microsomal; xanthine oxidase and dehydrogenase-soluble phase, and NADP: isocitrate dehydrogenase-soluble phase and mitochondrial. 3. 3. Alkaline phosphatase is confined to brush borders and is absent from the basolateral plasma membrane. A variable proportion of the activity, up to 40% is on brush borders which during homogenization break up into particles of reduced density and slow sedimentation rate.

Original languageEnglish
Pages (from-to)327-332
Number of pages6
JournalComparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology
Volume67
Issue number2
DOIs
Publication statusPublished - 1980

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