TY - JOUR
T1 - Subcellular localization of monoglyceride acyltransferase, xanthine oxidation, NADP
T2 - Isocitrate dehydrogenase and alkaline phosphatase in the mucosa of the guinea-pig small intestine
AU - Martin, P. A.
AU - Temple, N. J.
AU - Connock, M. J.
N1 - Funding Information:
Acknowledgement We gratefully acknowledge the financial support of the Wellcome Trust.
PY - 1980
Y1 - 1980
N2 - 1. 1. Rate dependent and isopycnic banding in a zonal rotor were used to analyse the subcellular sites of enzymes in homogenates of guinea-pig small intestinal mucosa. 2. 2. The results demonstrate the following localizations: monoglyceride acyltransferase-microsomal; xanthine oxidase and dehydrogenase-soluble phase, and NADP: isocitrate dehydrogenase-soluble phase and mitochondrial. 3. 3. Alkaline phosphatase is confined to brush borders and is absent from the basolateral plasma membrane. A variable proportion of the activity, up to 40% is on brush borders which during homogenization break up into particles of reduced density and slow sedimentation rate.
AB - 1. 1. Rate dependent and isopycnic banding in a zonal rotor were used to analyse the subcellular sites of enzymes in homogenates of guinea-pig small intestinal mucosa. 2. 2. The results demonstrate the following localizations: monoglyceride acyltransferase-microsomal; xanthine oxidase and dehydrogenase-soluble phase, and NADP: isocitrate dehydrogenase-soluble phase and mitochondrial. 3. 3. Alkaline phosphatase is confined to brush borders and is absent from the basolateral plasma membrane. A variable proportion of the activity, up to 40% is on brush borders which during homogenization break up into particles of reduced density and slow sedimentation rate.
UR - http://www.scopus.com/inward/record.url?scp=33646282262&partnerID=8YFLogxK
U2 - 10.1016/0305-0491(80)90152-2
DO - 10.1016/0305-0491(80)90152-2
M3 - Journal Article
AN - SCOPUS:33646282262
SN - 0305-0491
VL - 67
SP - 327
EP - 332
JO - Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology
IS - 2
ER -