TY - JOUR
T1 - Metal biosorption in lignocellulosic biofuel biorefinery effluent
T2 - An initial step towards sustainability of water resources
AU - Palumbo, Amanda J.
AU - Taylor, Sean C.
AU - Addison, Sarah L.
AU - Slade, Alison H.
AU - Glover, Chris N.
N1 - Funding Information:
We thank Sally Gaw and Rob Stainthorpe (University of Canterbury) for metals analysis; Matt Stott and Chris Daughney (Institute of Geological and Nuclear Sciences, GNS) for valuable scientific input; Phil Novis (Landcare Research) for providing Scenedesmus sp. and Chlorella sp.; Susie Wood (Cawthron Research) for providing Microcystis wesenbergii and other algae; Tripti Singh (Scion, Rotorua) for providing the Trichoderma viride, Trametes versicolor and Antrodia xantha; Ben MacDonald and Katrin Walbert (Scion). Funding provided by the Foundation for Research, Science and Technology (FRST) programme CO4 × 0801.
PY - 2012/9
Y1 - 2012/9
N2 - Biosorption of metals by microorganisms is a promising technology to remove accumulated non-process elements in highly recycled biorefinery process water. Removal of these elements would enable greater water reuse and reduce the environmental impact of effluent discharge. A model lignocellulosic ethanol biorefinery wastewater was created based on pulp mill effluent. This generated a wastewater with an environmentally realistic high loading of dissolved natural organic matter (900 mg/l), a potentially important factor influencing metal biosorption. Analysis of feedstock and pulp mill effluent indicated that Mn and Zn are likely to be problematic in highly recycled lignocellulosic ethanol biorefinery process water. Therefore, the growth of several bacteria and fungi from existing collections, and some isolated from pulp mill effluent were tested in the model wastewater spiked with Mn and Zn (0.2 mM). Wastewater isolates grew the best in the wastewater. Metal uptake varied by species and was much greater for Zn than Mn. A bacterium, Novosphingobium nitrogenifigens Y88T, removed the most metal per unit biomass, 35 and 17 mg Mn/g. No other organism tested decreased the Mn concentration. A yeast, Candida tropicalis, produced the most biomass and removed the most total metal (38 % of Zn), while uptake per unit biomass was 24 mg Zn/g. These results indicate that microorganisms can remove significant amounts of metals in wastewater with high concentrations of dissolved natural organic matter. Metal sorption by autochthonous microorganisms in an anaerobic bioreactor may be able to extend water reuse and therefore lower the water consumption of future biorefineries.
AB - Biosorption of metals by microorganisms is a promising technology to remove accumulated non-process elements in highly recycled biorefinery process water. Removal of these elements would enable greater water reuse and reduce the environmental impact of effluent discharge. A model lignocellulosic ethanol biorefinery wastewater was created based on pulp mill effluent. This generated a wastewater with an environmentally realistic high loading of dissolved natural organic matter (900 mg/l), a potentially important factor influencing metal biosorption. Analysis of feedstock and pulp mill effluent indicated that Mn and Zn are likely to be problematic in highly recycled lignocellulosic ethanol biorefinery process water. Therefore, the growth of several bacteria and fungi from existing collections, and some isolated from pulp mill effluent were tested in the model wastewater spiked with Mn and Zn (0.2 mM). Wastewater isolates grew the best in the wastewater. Metal uptake varied by species and was much greater for Zn than Mn. A bacterium, Novosphingobium nitrogenifigens Y88T, removed the most metal per unit biomass, 35 and 17 mg Mn/g. No other organism tested decreased the Mn concentration. A yeast, Candida tropicalis, produced the most biomass and removed the most total metal (38 % of Zn), while uptake per unit biomass was 24 mg Zn/g. These results indicate that microorganisms can remove significant amounts of metals in wastewater with high concentrations of dissolved natural organic matter. Metal sorption by autochthonous microorganisms in an anaerobic bioreactor may be able to extend water reuse and therefore lower the water consumption of future biorefineries.
KW - Bioremediation
KW - Biosorption
KW - Lignocellulosic ethanol biorefinery
KW - Metal
KW - Organic matter
UR - http://www.scopus.com/inward/record.url?scp=84867337979&partnerID=8YFLogxK
U2 - 10.1007/s10295-012-1129-6
DO - 10.1007/s10295-012-1129-6
M3 - Journal Article
C2 - 22535223
AN - SCOPUS:84867337979
SN - 1367-5435
VL - 39
SP - 1345
EP - 1356
JO - Journal of Industrial Microbiology and Biotechnology
JF - Journal of Industrial Microbiology and Biotechnology
IS - 9
ER -