TY - JOUR
T1 - Layer-by-layer assembly of epidermal growth factors on polyurethane films for wound closure
AU - Kulkarni, Abhilash
AU - Diehl-Jones, William
AU - Ghanbar, Sadegh
AU - Liu, Song
N1 - Funding Information:
This work was supported by the Manitoba Health Research Council (MHRC) Operating grant, the Collaborative Health Research Projects (CHRP) operating grant (Grant no.: CHRP 413713-2012), and the Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery grant (Grant no.: RGPIN/372048-2009). Acknowledgements
PY - 2014/8
Y1 - 2014/8
N2 - To facilitate the healing of chronic wounds, growth factors such as epidermal growth factor need to be safely encapsulated for their sustained and effective delivery to the wound bed. Using a layer-by-layer assembly technique, epidermal growth factor is successfully encapsulated on the surface of poly(acrylic acid)-modified polyurethane film. The amount of encapsulated epidermal growth factor is controlled by adjusting the number of chitosan/epidermal growth factor bilayers. A controlled release of epidermal growth factor from the surface of polyurethane film for a period of five days is achieved with well-retained bioactivity (over 90%) as evidenced by a cell proliferation assay. In an in vitro cellular wounding assay, the cell gap covered with the epidermal growth factor-loaded polyurethane film closes at a rate more than twice as fast as that covered with a control polyurethane film. Fluorescent staining of F-actin reveals that the released epidermal growth factor induces differences in cytoskeletal organization, suggesting that stimulated cell migration also contributes to the close of the cell gap.
AB - To facilitate the healing of chronic wounds, growth factors such as epidermal growth factor need to be safely encapsulated for their sustained and effective delivery to the wound bed. Using a layer-by-layer assembly technique, epidermal growth factor is successfully encapsulated on the surface of poly(acrylic acid)-modified polyurethane film. The amount of encapsulated epidermal growth factor is controlled by adjusting the number of chitosan/epidermal growth factor bilayers. A controlled release of epidermal growth factor from the surface of polyurethane film for a period of five days is achieved with well-retained bioactivity (over 90%) as evidenced by a cell proliferation assay. In an in vitro cellular wounding assay, the cell gap covered with the epidermal growth factor-loaded polyurethane film closes at a rate more than twice as fast as that covered with a control polyurethane film. Fluorescent staining of F-actin reveals that the released epidermal growth factor induces differences in cytoskeletal organization, suggesting that stimulated cell migration also contributes to the close of the cell gap.
KW - Delivery of EGF
KW - LbL assembly
KW - PU film
KW - wound healing
UR - http://www.scopus.com/inward/record.url?scp=84904365590&partnerID=8YFLogxK
U2 - 10.1177/0885328214523058
DO - 10.1177/0885328214523058
M3 - Journal Article
AN - SCOPUS:84904365590
SN - 0885-3282
VL - 29
SP - 278
EP - 290
JO - Journal of Biomaterials Applications
JF - Journal of Biomaterials Applications
IS - 2
ER -