Layer-by-layer assembly of epidermal growth factors on polyurethane films for wound closure

Abhilash Kulkarni, William Diehl-Jones, Sadegh Ghanbar, Song Liu

    Research output: Contribution to journalJournal Articlepeer-review

    18 Citations (Scopus)

    Abstract

    To facilitate the healing of chronic wounds, growth factors such as epidermal growth factor need to be safely encapsulated for their sustained and effective delivery to the wound bed. Using a layer-by-layer assembly technique, epidermal growth factor is successfully encapsulated on the surface of poly(acrylic acid)-modified polyurethane film. The amount of encapsulated epidermal growth factor is controlled by adjusting the number of chitosan/epidermal growth factor bilayers. A controlled release of epidermal growth factor from the surface of polyurethane film for a period of five days is achieved with well-retained bioactivity (over 90%) as evidenced by a cell proliferation assay. In an in vitro cellular wounding assay, the cell gap covered with the epidermal growth factor-loaded polyurethane film closes at a rate more than twice as fast as that covered with a control polyurethane film. Fluorescent staining of F-actin reveals that the released epidermal growth factor induces differences in cytoskeletal organization, suggesting that stimulated cell migration also contributes to the close of the cell gap.

    Original languageEnglish
    Pages (from-to)278-290
    Number of pages13
    JournalJournal of Biomaterials Applications
    Volume29
    Issue number2
    DOIs
    Publication statusPublished - Aug. 2014

    Keywords

    • Delivery of EGF
    • LbL assembly
    • PU film
    • wound healing

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