Intestinal peroxisomes of goldfish (Carassius auratus)-examination for hydrolase, dehydrogenase and carnitine acetyltransferase activities

N. J. Temple; P. A. Martin; M. J. Connock

doi:10.1016/0305-0491(79)90183-4

Intestinal peroxisomes of goldfish (Carassius auratus)-examination for hydrolase, dehydrogenase and carnitine acetyltransferase activities

N. J. Temple, P. A. Martin, M. J. Connock

Centre for Science

University of Wolverhampton

Research output: Contribution to journal › Journal Article › peer-review

5 Citations (Scopus)

Abstract

1. 1. Rate sedimentation and isopycnic centrifugation were used to analyse the subcellular sites of enzymes in homogenates of goldfish intestinal mucosa. 2. 2. The results allowed the following allocations to be made: carnitine acetyl transferase-mitochondrial and peroxisomal, xanthine dehydrogenase and NAD: α-glycerophosphate dehydrogenase soluble phase, NADP: isocitrate dehydrogenase soluble phase and mitochondrial, and 2-naphthyl laurate hydrolase microsomal and/or brush border. 3. 3. Histochemistry confirmed the use of alkaline phosphatase and 1-naphthyl acetate esterase as brush border and microsome markers respectively. 4. 4. Urate oxidase, allantomase, allantoicase, xanthine oxidase and glycollate/lactate oxidase, activities were undetectable, and 1-naphthyl palmitate hydrolase was present only as a contaminant from pancreas.

Original language	English
Pages (from-to)	57-63
Number of pages	7
Journal	Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology
Volume	64
Issue number	1
DOIs	https://doi.org/10.1016/0305-0491(79)90183-4
Publication status	Published - 1979

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title = "Intestinal peroxisomes of goldfish (Carassius auratus)-examination for hydrolase, dehydrogenase and carnitine acetyltransferase activities",

abstract = "1. 1. Rate sedimentation and isopycnic centrifugation were used to analyse the subcellular sites of enzymes in homogenates of goldfish intestinal mucosa. 2. 2. The results allowed the following allocations to be made: carnitine acetyl transferase-mitochondrial and peroxisomal, xanthine dehydrogenase and NAD: α-glycerophosphate dehydrogenase soluble phase, NADP: isocitrate dehydrogenase soluble phase and mitochondrial, and 2-naphthyl laurate hydrolase microsomal and/or brush border. 3. 3. Histochemistry confirmed the use of alkaline phosphatase and 1-naphthyl acetate esterase as brush border and microsome markers respectively. 4. 4. Urate oxidase, allantomase, allantoicase, xanthine oxidase and glycollate/lactate oxidase, activities were undetectable, and 1-naphthyl palmitate hydrolase was present only as a contaminant from pancreas.",

author = "Temple, {N. J.} and Martin, {P. A.} and Connock, {M. J.}",

year = "1979",

doi = "10.1016/0305-0491(79)90183-4",

language = "English",

volume = "64",

pages = "57--63",

number = "1",

}

Intestinal peroxisomes of goldfish (Carassius auratus)-examination for hydrolase, dehydrogenase and carnitine acetyltransferase activities. / Temple, N. J.; Martin, P. A.; Connock, M. J.
In: Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology, Vol. 64, No. 1, 1979, p. 57-63.

Research output: Contribution to journal › Journal Article › peer-review

TY - JOUR

T1 - Intestinal peroxisomes of goldfish (Carassius auratus)-examination for hydrolase, dehydrogenase and carnitine acetyltransferase activities

AU - Temple, N. J.

AU - Martin, P. A.

AU - Connock, M. J.

PY - 1979

Y1 - 1979

N2 - 1. 1. Rate sedimentation and isopycnic centrifugation were used to analyse the subcellular sites of enzymes in homogenates of goldfish intestinal mucosa. 2. 2. The results allowed the following allocations to be made: carnitine acetyl transferase-mitochondrial and peroxisomal, xanthine dehydrogenase and NAD: α-glycerophosphate dehydrogenase soluble phase, NADP: isocitrate dehydrogenase soluble phase and mitochondrial, and 2-naphthyl laurate hydrolase microsomal and/or brush border. 3. 3. Histochemistry confirmed the use of alkaline phosphatase and 1-naphthyl acetate esterase as brush border and microsome markers respectively. 4. 4. Urate oxidase, allantomase, allantoicase, xanthine oxidase and glycollate/lactate oxidase, activities were undetectable, and 1-naphthyl palmitate hydrolase was present only as a contaminant from pancreas.

AB - 1. 1. Rate sedimentation and isopycnic centrifugation were used to analyse the subcellular sites of enzymes in homogenates of goldfish intestinal mucosa. 2. 2. The results allowed the following allocations to be made: carnitine acetyl transferase-mitochondrial and peroxisomal, xanthine dehydrogenase and NAD: α-glycerophosphate dehydrogenase soluble phase, NADP: isocitrate dehydrogenase soluble phase and mitochondrial, and 2-naphthyl laurate hydrolase microsomal and/or brush border. 3. 3. Histochemistry confirmed the use of alkaline phosphatase and 1-naphthyl acetate esterase as brush border and microsome markers respectively. 4. 4. Urate oxidase, allantomase, allantoicase, xanthine oxidase and glycollate/lactate oxidase, activities were undetectable, and 1-naphthyl palmitate hydrolase was present only as a contaminant from pancreas.

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U2 - 10.1016/0305-0491(79)90183-4

DO - 10.1016/0305-0491(79)90183-4

M3 - Journal Article

C2 - 318295

AN - SCOPUS:0018657569

SN - 0305-0491

VL - 64

SP - 57

EP - 63

JO - Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology

JF - Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology

IS - 1

ER -

Intestinal peroxisomes of goldfish (Carassius auratus)-examination for hydrolase, dehydrogenase and carnitine acetyltransferase activities

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