TY - JOUR
T1 - Field-to-laboratory transport protocol impacts subsequent physiological biomarker response in the marine mussel, Perna canaliculus
AU - Chandurvelan, Rathishri
AU - Marsden, Islay D.
AU - Gaw, Sally
AU - Glover, Chris N.
N1 - Funding Information:
We are grateful to Dr. Andrea M. Contreras for guidance with the physiological measurements, Rennie Bishop for assistance with mussel collection, and Drs. Jonathan Hill, Elena Moltchanova and Mike Hickford for statistical support. This study was aided by a research grant from the Brian Mason Scientific and Technical Trust . RC is grateful for the support provided by a University of Canterbury Doctoral Scholarship .
PY - 2013
Y1 - 2013
N2 - The transfer of mussels from field to laboratory, or transplantation between clean and contaminated field settings, is a common protocol in ecotoxicology. However, collection and transport of mussels could lead to stress that may impact biomarker responses, and thus confound interpretation of results. Physiological responses (clearance rate, absorption efficiency, excretion rate, respiration rate and scope-for-growth) of green-lipped mussels (Perna canaliculus) exposed to four different transportation protocols were investigated. These protocols included immersion in site seawater (SSW), immersion in artificial seawater (ASW), and emersion (aerial transport; EMS) at two temperatures (15. °C and 5. °C). Physiological measurements were conducted after a simulated 24. h "transport" phase and a 48. h "recovery" phase. Clearance rates were significantly inhibited by the EMS 5. °C and ASW protocols relative to SSW treatment, although the clearance rate of the latter recovered after 48. h. A similar pattern was observed for excretion and respiration rates for ASW. Decreased excretion rates for EMS 15. °C and respiration rates for EMS 5. °C were also recorded relative to values for SSW following "recovery". Negative scope-for-growth was observed for all treatments except EMS 15. °C. These data suggest transport emersed at ambient air temperatures is the best method to maintain physiological health of green-lipped mussels.
AB - The transfer of mussels from field to laboratory, or transplantation between clean and contaminated field settings, is a common protocol in ecotoxicology. However, collection and transport of mussels could lead to stress that may impact biomarker responses, and thus confound interpretation of results. Physiological responses (clearance rate, absorption efficiency, excretion rate, respiration rate and scope-for-growth) of green-lipped mussels (Perna canaliculus) exposed to four different transportation protocols were investigated. These protocols included immersion in site seawater (SSW), immersion in artificial seawater (ASW), and emersion (aerial transport; EMS) at two temperatures (15. °C and 5. °C). Physiological measurements were conducted after a simulated 24. h "transport" phase and a 48. h "recovery" phase. Clearance rates were significantly inhibited by the EMS 5. °C and ASW protocols relative to SSW treatment, although the clearance rate of the latter recovered after 48. h. A similar pattern was observed for excretion and respiration rates for ASW. Decreased excretion rates for EMS 15. °C and respiration rates for EMS 5. °C were also recorded relative to values for SSW following "recovery". Negative scope-for-growth was observed for all treatments except EMS 15. °C. These data suggest transport emersed at ambient air temperatures is the best method to maintain physiological health of green-lipped mussels.
KW - Aquaculture
KW - Biomarkers
KW - Perna canaliculus
KW - Physiology
KW - Scope-for-growth
KW - Transplantation
KW - Transportation
UR - http://www.scopus.com/inward/record.url?scp=84870187209&partnerID=8YFLogxK
U2 - 10.1016/j.cbpa.2012.10.011
DO - 10.1016/j.cbpa.2012.10.011
M3 - Journal Article
C2 - 23085018
AN - SCOPUS:84870187209
SN - 1095-6433
VL - 164
SP - 84
EP - 90
JO - Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology
JF - Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology
IS - 1
ER -
