TY - JOUR
T1 - Effect of high fat and nutrient depleted diets on colon tumor formation in mice
AU - Temple, Norman J.
AU - El-Khatib, Shukri M.
N1 - Funding Information:
The authors thank Mrs. Myrna Nieves for skillful technical assistance, Mrs. Mabel Baaske and Mrs. Sandra Casovan for histological work and Dr. Lawrence Jewel1 (Department of Pathology, University Hospital, Edmonton, Canada) for advice on the interpretation of colon tumors. We also thank The Alberta Heritage Foundation for Medical Research for financial support.
PY - 1987/10
Y1 - 1987/10
N2 - We investigated the effect of high fat and nutrient depleted diets on the formation of colon tumors in female Swiss mice treated with 1,2-dimethylhydrazine (DMH). Mice received the following diets: control diet (laboratory chow, 5.5% fat) or chow with added starch and/or corn oil so as to supply a fat level of 5.5% (diet F6) or 23% (diet F23), while reducing the level of nutrients and dietary fiber per 100 calories to 61% of the level of the control diet. DMH was given as 5 weekly s.c. injections. Diets were given variously from 6 weeks before the first injection until 7 days after the last one (initiation period) or else for the subsequent 22 weeks until sacrifice of the mice (promotion period). A high fat diet (diet F23 vs. F6) tended to increase adenoma formation when fed during the initiation period but this requires further study. Feeding it in the promotion period increased the incidence of adenocarcinomas. A nutrient depleted diet (diet F6 vs. the control diet) caused a decreased incidence of adenocarcinomas.
AB - We investigated the effect of high fat and nutrient depleted diets on the formation of colon tumors in female Swiss mice treated with 1,2-dimethylhydrazine (DMH). Mice received the following diets: control diet (laboratory chow, 5.5% fat) or chow with added starch and/or corn oil so as to supply a fat level of 5.5% (diet F6) or 23% (diet F23), while reducing the level of nutrients and dietary fiber per 100 calories to 61% of the level of the control diet. DMH was given as 5 weekly s.c. injections. Diets were given variously from 6 weeks before the first injection until 7 days after the last one (initiation period) or else for the subsequent 22 weeks until sacrifice of the mice (promotion period). A high fat diet (diet F23 vs. F6) tended to increase adenoma formation when fed during the initiation period but this requires further study. Feeding it in the promotion period increased the incidence of adenocarcinomas. A nutrient depleted diet (diet F6 vs. the control diet) caused a decreased incidence of adenocarcinomas.
UR - http://www.scopus.com/inward/record.url?scp=0023640593&partnerID=8YFLogxK
U2 - 10.1016/0304-3835(87)90151-0
DO - 10.1016/0304-3835(87)90151-0
M3 - Journal Article
C2 - 3664447
AN - SCOPUS:0023640593
SN - 0304-3835
VL - 37
SP - 109
EP - 114
JO - Cancer Letters
JF - Cancer Letters
IS - 1
ER -
