@inproceedings{05a1c2af76274d219adf2f0bdee4e053,
title = "A method for assessing kinetic changes of histone hi after post-translational modifications",
abstract = "Fluorescence Recovery After Photobleaching (FRAP) has become one of the main fluorescence microscopy techniques used to measure motion and interaction of nuclear proteins. In this work, we propose a mathematical method for analyzing sets of FRAP data to assess the effect of post-translational modifications on the binding affinities of histone HI proteins. The method is based on a model that accounts for weakly and strongly bound populations and comprises the estimation of kinetic parameters before and after post-translational modifications and the use of non-parametric statistics to analyze changes in the estimated parameters. The procedure is illustrated with sets of FRAP data of histone HI binding to hyperacetylated chromatin.",
keywords = "Binding affinity, Fluorescence Recovery After Photobleaching (FRAP), Histone HI, Post-translational modifications",
author = "Gustavo Carrero and Nikhil Raghuram and John Th'Ng and Michael Hendzel",
year = "2009",
doi = "10.1063/1.3241319",
language = "English",
isbn = "9780735407091",
series = "AIP Conference Proceedings",
pages = "1306--1309",
booktitle = "Numerical Analysis and Applied Mathematics - International Conference on Numerical Analysis and Applied Mathematics 2009, ICNAAM-2009",
note = "International Conference on Numerical Analysis and Applied Mathematics 2009, ICNAAM-2009 ; Conference date: 18-09-2009 Through 22-09-2009",
}