A method for assessing kinetic changes of histone hi after post-translational modifications

Gustavo Carrero, Nikhil Raghuram, John Th'Ng, Michael Hendzel

Research output: Chapter in Book/Report/Conference proceedingPublished Conference contributionpeer-review

2 Citations (Scopus)

Abstract

Fluorescence Recovery After Photobleaching (FRAP) has become one of the main fluorescence microscopy techniques used to measure motion and interaction of nuclear proteins. In this work, we propose a mathematical method for analyzing sets of FRAP data to assess the effect of post-translational modifications on the binding affinities of histone HI proteins. The method is based on a model that accounts for weakly and strongly bound populations and comprises the estimation of kinetic parameters before and after post-translational modifications and the use of non-parametric statistics to analyze changes in the estimated parameters. The procedure is illustrated with sets of FRAP data of histone HI binding to hyperacetylated chromatin.

Original languageEnglish
Title of host publicationNumerical Analysis and Applied Mathematics - International Conference on Numerical Analysis and Applied Mathematics 2009, ICNAAM-2009
Pages1306-1309
Number of pages4
DOIs
Publication statusPublished - 2009
EventInternational Conference on Numerical Analysis and Applied Mathematics 2009, ICNAAM-2009 - Rethymno, Crete, Greece
Duration: 18 Sep. 200922 Sep. 2009

Publication series

NameAIP Conference Proceedings
Volume1168
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

ConferenceInternational Conference on Numerical Analysis and Applied Mathematics 2009, ICNAAM-2009
Country/TerritoryGreece
CityRethymno, Crete
Period18/09/0922/09/09

Keywords

  • Binding affinity
  • Fluorescence Recovery After Photobleaching (FRAP)
  • Histone HI
  • Post-translational modifications

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